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In Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
In Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice

In Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice

DOI:
10.3791/60617-v

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06:57 min

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February 20, 2020

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, , , ,
1Department of Physiology, Institute of Bioscience,University of Sao Paulo

Capítulos

  • 00:04Introduction
  • 01:16In Vitro Differentiation of L. amazonensis Promastigotes into Axenic Amastigotes
  • 01:56C57BL/6 Footpad Infection with L. amazonensis
  • 02:47Mouse Footpad Lesion Extraction and Parasite Load Determination
  • 04:29Results: LIR1 Knockout In Vivo Lesion Development and Intracellular Replication
  • 06:03Conclusion

Summary

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Here, we present a compiled protocol to evaluate the cutaneous infection of mice with Leishmania amazonensis. This is a reliable method for studying parasite virulence, allowing a systemic view of the vertebrate host response to the infection.

Tags

Keywords: Leishmania AmazonensisIn Vivo InfectionVirulenceCutaneous LeishmaniasisMouse ModelPromastigotesAmastigotesSubplantar InjectionLesion ProgressionParasite Quantification

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