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Analysis of Side Population in Solid Tumor Cell Lines
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JoVE Journal Pesquisa do Câncer
Analysis of Side Population in Solid Tumor Cell Lines

Analysis of Side Population in Solid Tumor Cell Lines

DOI:
10.3791/60658-v

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06:26 min

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February 23, 2021

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, , , ,
1School of Medicine,Nankai University, 2Tianjin Key Laboratory of Tumour Microenvironment and Neurovascular Regulation, 3Collaborative Innovation Center for Biotherapy,Nankai University

Capítulos

  • 00:04Introduction
  • 00:51Cell Preparation
  • 02:02Cell Staining
  • 03:28Flow Cytometry
  • 04:12Results: SP Analysis
  • 05:54Conclusion

Summary

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A convenient, fast, and cost-effective method to measure the proportion of side population cells in solid tumor cell lines is presented.

Tags

Side PopulationCancer Stem CellsTumor Cell LinesHoechst 33342Flow CytometrySP AnalysisStemness PropertiesCell CultureCell Preparation

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