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Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore
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JoVE Journal Bioquímica
Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore

Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore

DOI:
10.3791/60659-v

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09:15 min

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January 21, 2020

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,
1Biomedical Engineering Program,Ohio University, 2Department of Chemical and Biomolecular Engineering,Ohio University

Capítulos

  • 00:04Título
  • 00:42Erythrocyte Isolation from Whole Blood
  • 02:19Treatment of Erythrocytes with Ionomycin and Measurement of Hemolysis
  • 04:43Annexin-V Binding Assay
  • 05:48Flow Cytometry
  • 06:47Results: Flow Cytometry and Confocal Fluorescence Microscopy
  • 08:12Conclusion

Summary

Tadução automática

Tadução automática

A protocol for the induction of eryptosis, programmed cell death in erythrocytes, using the calcium ionophore, ionomycin, is provided. Successful eryptosis is evaluated by monitoring the localization phosphatidylserine in the membrane outer leaflet. Factors affecting the success of the protocol have been examined and optimal conditions provided.

Tags

Keywords: EryptosisRed Blood CellsCalcium IonophoreIonomycinRinger SolutionGlucose-free BufferHematocritCell SuspensionCentrifugationIncubation

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