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Preparation of Meiotic Chromosome Spreads from Zebrafish Spermatocytes
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JoVE Journal Genética
Preparation of Meiotic Chromosome Spreads from Zebrafish Spermatocytes

Preparation of Meiotic Chromosome Spreads from Zebrafish Spermatocytes

DOI:
10.3791/60671-v

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08:46 min

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March 03, 2020

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, ,
1Department of Molecular and Cellular Biology,University of California, Davis, 2Integrative Genetics and Genomics Graduate Group,University of California, Davis

Capítulos

  • 00:05Introduction
  • 00:54Dissection of Testes from Adult Male Zebrafish
  • 07:12Results: Chromosome Spreads
  • 07:51Conclusion

Summary

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Nuclear surface spreads are an indispensable tool for studying chromosome events during meiosis. Here we demonstrate a method to prepare and visualize meiotic chromosomes during prophase I from zebrafish spermatocytes.

Tags

Meiotic Chromosome SpreadsZebrafish SpermatocytesVertebrate ModelMeiosisHomologous Chromosome PairingSynapsisRecombinationSuper Resolution MicroscopyDissectionTestesCollagenaseDMEMSucrose SolutionCell Dissociation

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