Journal
/
/
通过四氯化碳诱导大鼠急性肝损伤 (CCl4) 通过奥罗胃管暴露
JoVE Journal
Medicina
Author Produced
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Medicina
Inducing Acute Liver Injury in Rats via Carbon Tetrachloride (CCl4) Exposure Through an Orogastric Tube
DOI:

06:12 min

April 28, 2020

, , , , , , , , ,

Capítulos

  • 00:03Introduction
  • 00:23Preparing Rats for the Experimental Procedure
  • 01:15Determination of Serum GOT, GPT and Total Bilirubin Baseline Level
  • 01:44The Procedure for Induction of Acute Liver Injury in Rats
  • 03:03Determination of Serum GOT, GPT and Total Bilirubin Level after 24 Hours
  • 03:23Liver Collection for Histological Examination
  • 04:03Histological Examination on Rats at Time 24 Hours
  • 04:49Resultados
  • 05:38Conclusion

Summary

Tadução automática

该协议描述了通过CCl4暴露通过奥胃管诱导急性肝损伤(ALI)的一种常见和可行的方法。CCl4暴露在肝脏生物转化过程中通过活性氧物种的形成诱导 ALI。该方法用于分析ALI的病理生理学,并研究不同的肝保护策略。

Vídeos Relacionados

Read Article