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Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales
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Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales

Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales

DOI:
10.3791/60701-v

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12:32 min

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November 25, 2020

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, , ,
1Stream Biofilm and Ecosystem Research Laboratory,École Polytechnique Fédérale de Lausanne, 2Institute of Earth Sciences,University of Lausanne, CH-1015

Capítulos

  • 00:04Introduction
  • 00:57Preparation of A Microfluidic Device in Polydimethylsiloxane (PDMS)
  • 03:27Preparation of a Fluidic Device in Poly(methyl methacrylate)(PMMA)
  • 04:09Analyze Bacterial Transport using PDMS Microfluidic Devices
  • 05:10Basic Image Processing
  • 06:59Analyze Bacterial Transport at the Pore Scale
  • 08:08Study Bacterial Filtration by Means of Deposition Profiles
  • 08:51Analyze Bacterial Transport using PMMA Fluidic Devices
  • 10:33Results: Motile and Nonmotile GFP-tagged P. putida KT2440
  • 11:48Conclusion

Summary

Tadução automática

Tadução automática

Breakthrough curves (BTCs) are efficient tools to study the transport of bacteria in porous media. Here we introduce tools based on fluidic devices in combination with microscopy and flow cytometric counting to obtain BTCs.

Tags

Keywords: Microfluidic DevicesMicroscopyFlow CytometryBacterial TransportPorous MediaSpatial ScalesBioremediationContaminationDisease SpreadMathematical ModelsSingle CellPopulationMicrobial CommunityPDMSSU-8 PhotolithographyPorous GeometryFluidic DevicesAutomated DispenserLaboratory-scale Column StudiesField Surveys

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