Ubiquitin Chain Analysis by Parallel Reaction Monitoring

Journal

Bioquímica

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Ubiquitin Chain Analysis by Parallel Reaction Monitoring

DOI:

10.3791/60702-v

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08:33 min

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June 17, 2020

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Joseph Longworth, Marta L. Mendes, Gunnar Dittmar

¹Quantitative Biology Unit,Luxembourg Institute of Health

Capítulos

00:05Introduction
00:51Sample Preparation
04:04Software Analysis
05:49Results: Derivation of the Ubiquitin Topology-characteristic Peptide
07:44Conclusion

Summary

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This method describes the assessment of global changes in ubiquitin chain topology. The assessment is performed by the application of a mass spectrometry-based targeted proteomics approach.

Ubiquitin Chain Analysis by Parallel Reaction Monitoring

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Capítulos

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