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完全加工重组KRAS4b：分离和描述脱脂和甲基化蛋白

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Fully Processed Recombinant KRAS4b: Isolating and Characterizing the Farnesylated and Methylated Protein

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完全加工重组KRAS4b：分离和描述脱脂和甲基化蛋白

DOI:

10.3791/60703-v

•

07:08 min

•

January 16, 2020

•

Constance Agamasu, Peter Frank, Shelley Perkins, Timothy Waybright, Simon Messing, William Gillette, Andrew G. Stephen

¹NCI RAS Initiative, Cancer Research Technology Program,Frederick National Laboratory for Cancer Research

Capítulos

00:05Título
01:30Protein Purification
04:20Results: Purification and Analysis of KRAS4b Proteins
06:14Conclusion

Summary

Tadução automática

前膜化是外周膜结合蛋白的重要修饰。昆虫细胞可以纵产生脱脂和甲苯甲酸化 KRAS4b 的数量，使生物物理测量蛋白质 - 蛋白质和蛋白质 - 脂质相互作用

Tags

KRAS4b Farnesylation Methylation Recombinant Protein Protein Purification Cation Exchange Chromatography Baculovirus Expression Cancer Biophysical Experiments

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