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肝肿瘤细胞背景下自然杀伤细胞介导细胞毒性和迁移的测量
JoVE Journal
Medicina
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JoVE Journal Medicina
Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells
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肝肿瘤细胞背景下自然杀伤细胞介导细胞毒性和迁移的测量

DOI:
10.3791/60714-v

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06:55 min

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February 22, 2020

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, , ,
1Department of Biochemistry and Molecular Genetics,University of Alabama at Birmingham

Capítulos

  • 00:05Introduction
  • 00:52Colorimetric Lactic Dehydrogenase (LDH) Measurement-Based Natural Killer (NK) Cell-Mediated Cytotoxicity Assay
  • 02:46Calcein AM Staining-Based NK Cell-Mediated Cytotoxicity Assay
  • 04:10NK Cell Migration Assay
  • 05:26Results: Representative NK Cell-Mediated Cytoxicity and Migration
  • 06:17Conclusion

Summary

Tadução automática

Tadução automática

癌细胞对自然杀手（NK）细胞介导的根除的逃避对癌症的启动和进展非常重要。在这里，我们提出了两个非放射性为基础的协议，以评估NK细胞介导的细胞毒性对肝肿瘤细胞。此外，还提出了第三个协议来分析NK细胞迁移。

Tags

Keywords: NK Cell FunctionNK Cell-mediated CytotoxicityNK Cell MigrationTumor MicroenvironmentLDH AssayCalcein AM AssayHuman Liver Cancer CellsCell CultureCentrifugation96-well PlateAbsorbance MeasurementCytotoxicity Calculation

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