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Immunology and Infection

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JoVE Journal Immunology and Infection
Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection
 

Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection

Article DOI: 10.3791/60723-v • 08:15 min • January 31st, 2020
January 31st, 2020 •
1,2, 1,2, 1,2, 1, 3, 1,4, 1,2, 1,2, 1,2,5, 1,2
1Department of Clinical Medicine, Aarhus University, 2Department of Infectious Diseases, Aarhus University Hospital, 3Department of Biomedicine, Aarhus University, 4Department of Gynecology and Obstetrics, Aarhus University Hospital, 5Department of Biology, University of Nebraska at Omaha

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Resumo
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We have developed a protocol for the generation and evaluation of a humanized and human immunodeficiency virus-infected NOG mouse model based on stem cell transplant, intravaginal human immunodeficiency virus exposure, and droplet digital PCR RNA quantification.

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Keywords: Humanized NOG Mice Intravaginal HIV Exposure HIV Infection Preclinical HIV Research Viral Strains HIV Interventions CCR5 Variant Viral RNA Quantification Cord Blood Screening CCR5 Delta 32 HIV Exposure Protocol
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