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Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody

JoVE Journal
Pesquisa do Câncer

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JoVE Journal Pesquisa do Câncer

Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody

Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody

DOI:

10.3791/60727-v

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07:36 min

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May 16, 2020

•

Gururaj Shivange*^1,2, Tanmoy Mondal*^1,2, Evan Lyerly², Jeremy Gatesman, Jogender Tushir-Singh²

¹Laboratory of Novel Biologics,University of Virginia School of Medicine, ²Department of Biochemistry and Molecular Genetics, UVA Cancer Center,University of Virginia School of Medicine, ³Center for Comparative Medicine,University of Virginia

Capítulos

00:04Introduction
01:02Expression and Purification of Antibodies
03:22Fluorescent Labeling
04:10Antibody Localization Using an In Vivo Imaging System
06:05Results: Live Imaging of Human OVCAR3 Tumor Bearing Mice
06:56Conclusion

Summary

Tadução automática

Here we present a protocol to study the in vivo localization of antibodies in mice tumor xenograft models.

Tags

Keywords: Antibody Distribution Tumor Localization Antibody Screening CHO Cell Expression Antibody Purification Protein A Column Real-time Monitoring SPECT PET Cost-effective Technique

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