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TurboID-baseret nærhed mærkning for I Planta Identifikation af Protein-Protein Interaction Networks
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1State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences,China Agricultural University, 2Department of Plant Biology, College of Biological Sciences,University of California, Davis, 3Genome Center, College of Biological Sciences,University of California, Davis
Capítulos
- 00:04Introduction
- 01:01Agroinfiltration and Leaf Sample Collection
- 01:59Extraction of Leaf Total Protein and Removal of Free Biotin
- 04:11Enrichment of Biotinylated Proteins
- 05:53Results: Western Blot of Protein Extracts
- 06:25Conclusion
Beskrevet her er en nærhed mærkning metode til identifikation af interaktion partnere tir domæne NLR immunreceptoren i Nicotiana benthamiana bladvæv. Der findes også en detaljeret protokol til identifikation af interaktioner mellem andre proteiner af interesse ved hjælp af denne teknik i Nicotiana og andre plantearter.
