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免疫荧光和定量PCR对白蝇组织中白蝇病毒的定位和定量

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR

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免疫荧光和定量PCR对白蝇组织中白蝇病毒的定位和定量

DOI:

10.3791/60731-v

•

06:25 min

•

February 08, 2020

•

Fei-Xue Ban, Tian-Yan Yin, Qi Guo, Li-Long Pan, Yin-Quan Liu, Xiao-Wei Wang

¹Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogen and Insects, Institute of Insect Sciences,Zhejiang University

Capítulos

00:05Introduction
00:43Female Whitefly Midgut, Primary Salivary Gland (PSG), Ovary, and Hemolymph Dissection
02:03Tomato Yellow Leaf Curl Virus (TYLCV) Localization
03:40TYLCV Quantification
05:04Results: Representative TYLCV Detection and Quantification
05:46Conclusion

Summary

Tadução automática

我们描述了一种免疫荧光和定量PCR方法，用于昆虫组织中果子病毒的定位和定量。免疫荧光方案可用于共定病毒和载体蛋白。定量PCR协议可以扩展，以量化整个白蝇体内和受病毒感染的植物中的病毒。

Tags

Begomoviruses Whitefly Localization Quantification Immunofluorescence Quantitative PCR Virus-vector Interaction Virus Detection Virus Transmission Microscopy Virus Quantification

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