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使用光明场显微镜定量拉瓦尔斑马鱼的肝脏大小

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Quantifying Liver Size in Larval Zebrafish Using Brightfield Microscopy

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使用光明场显微镜定量拉瓦尔斑马鱼的肝脏大小

DOI:

10.3791/60744-v

•

07:35 min

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February 02, 2020

•

Srishti Kotiyal*¹, Alexis Fulbright*¹, Liam K. O’Brien*¹, Kimberley J. Evason

¹Department of Pathology, Department of Oncological Sciences, and Huntsman Cancer Institute,University of Utah School of Medicine

Capítulos

00:05Introduction
00:49Fixing Larvae and Dissecting Tissues Surrounding Liver
02:53Imaging
04:05Image Analysis
06:07Results: Zebrafish Larvae
06:53Conclusion

Summary

Tadução automática

在这里，我们演示了一种量化幼虫斑马鱼肝脏大小的方法，提供了一种评估遗传和药理操作对肝脏生长和发育的影响的方法。

Tags

Keywords: Liver Size Larval Zebrafish Brightfield Microscopy Organ Quantification Genetic Manipulation Pharmacologic Manipulation Liver Growth Liver Development Dissection In Situ Hybridization Confocal Imaging Euthanasia PBS PFA Skin Removal Yolk Removal Forceps Glass Dish

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