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Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes
DOI:

05:20 min

February 06, 2020

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Capítulos

  • 00:05Introduction
  • 00:35Explant Dissection and Cell Culture Procedure
  • 01:52Growth of Corneal Endothelial Cell Layers on Biomaterial Membranes
  • 03:28Results: Corneal Endothelial Cell Cultures Derived from Sheep and Human Explants
  • 04:47Conclusion

Summary

Tadução automática

This protocol describes the critical steps required to establish and grow corneal endothelial cell cultures from explants of human or sheep tissue. A method for subculturing corneal endothelial cells on membranous biomaterials is also presented.

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