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Crecimiento de capas celulares endoteliales corneales humanas y ovinas en membranas biomateriales
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1Queensland Eye Institute, 2King Faisal University, 3School of Biomedical Sciences, Faculty of Health,Queensland University of Technology, 4Institute of Health and Biomedical Innovation,Queensland University of Technology, 5Science and Engineering Faculty,Queensland University of Technology, 6Faculty of Medicine,University of Queensland, 7Australian Institute for Bioengineering and Nanotechnology,University of Queensland, 8Faculty of Science,University of Western Australia
Capítulos
- 00:05Introduction
- 00:35Explant Dissection and Cell Culture Procedure
- 01:52Growth of Corneal Endothelial Cell Layers on Biomaterial Membranes
- 03:28Results: Corneal Endothelial Cell Cultures Derived from Sheep and Human Explants
- 04:47Conclusion
Este protocolo describe los pasos críticos necesarios para establecer y cultivar cultivos celulares endoteliales corneales a partir de explantas de tejido humano o ovino. También se presenta un método para subculturizar células endoteliales corneales en biomateriales membranosas.
