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Kwantificering van ethanolniveaus in zebravisembryo's met behulp van head space gaschromatografie
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography
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Kwantificering van ethanolniveaus in zebravisembryo's met behulp van head space gaschromatografie

DOI:
10.3791/60766-v

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08:22 min

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February 11, 2020

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1Department of Biochemistry and Molecular Genetics, Alcohol Research Center,University of Louisville

Capítulos

  • 00:04Introduction
  • 00:58Measuring the Embryonic Volume Using Water Displacement
  • 02:14Treating Embryos with Ethanol
  • 02:50Processing Embryos for Head Space Gas Chromatography
  • 03:58Sample Measurements Using Gas Space Chromatography
  • 05:04Sample Ethanol Peak Integration and Concentration Analysis
  • 06:06Results: Ethanol Levels in Early Embryonic Zebrafish
  • 07:38Conclusion

Summary

Tadução automática

Tadução automática

Dit werk beschrijft een protocol om ethanolniveaus in een embryo van zebravissen te kwantificeren met behulp van hoofdruimtegaschromatografie van de juiste blootstellingsmethoden voor embryoverwerking en ethanolanalyse.

Tags

Keywords: EthanolZebrafishEmbryosGas ChromatographyPharmacokineticsChorionProteaseVolume MeasurementEmbryo ProcessingTime-course

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