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Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells
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Biologia
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JoVE Journal Biologia
Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells

DOI:
10.3791/60801-v

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10:21 min

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September 16, 2020

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, , ,
1Unité Mixte de Recherche 144 CNRS, Molecular Mechanisms of Intracellular Transport group,Institut Curie, 75005 Paris, France, 2PSL Research University, Paris, France, 3Sorbonne Université, Paris, France, 4INRIA, Université Paris-Sud, PSL

Capítulos

  • 00:04Introduction
  • 01:11Micropattern Preparation
  • 03:20Cell Seeding
  • 04:37Exocytosis Imaging
  • 05:16Exocytosis Coordinate Acquisition
  • 06:56Single Cell Spatial Analysis
  • 07:34Results: Representative Lysosomal Exocytosis and Spatial Analyses
  • 09:44Conclusion

Summary

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Live imaging of lysosomal exocytosis on micropatterned cells allows a spatial quantification of this process. Morphology normalization using micropatterns is an outstanding tool to uncover general rules about the spatial distribution of cellular processes.

Tags

Keywords: Micropatterned CellsExocytosisLive ImagingTOF MicroscopyCell SecretionMicropattern SurfacesCell DivisionSecretory EventsImmune RegulationCancerProteolytic EnzymesInvasionAntigen PresentationPolylysine Graft Polyethylene GlycolQuartz Photo MaskDeep UV LightWater DropletsMicropattern Imprints

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