Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / 使用冷冻胚胎进行转基因小鼠高效生产…
JoVE Journal
Genetics

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Genetics
使用冷冻胚胎进行转基因小鼠高效生产
 
Click here for the English version

使用冷冻胚胎进行转基因小鼠高效生产

Article DOI: 10.3791/60808-v • 06:46 min • April 2nd, 2020
April 2nd, 2020 •
*1,2,3, *4,5, 6,7, 8,9,10, 8,11, 3, 8,9,10,12,13, 1
1Max Planck Florida Institute for Neuroscience, 2Life Science Research Center, University of Toyama, 3Department of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, 4Graduate School of Innovative Life Science, University of Toyama, 5Department of Biochemistry, Faculty of Pharmacy, Cairo University, 6Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, 7Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology, Jichi Medical University, 8Synthetic Biology Division, Research Center for Advanced Science and Technology, University of Tokyo, 9Institute for Advanced Biosciences, Keio University, 10Graduate School of Media and Governance, Keio University, 11Department of Molecular Oncology, Graduate School of Medicine, Chiba University, 12Department of Biological Sciences, School of Science, University of Tokyo, 13College of Arts and Sciences, University of Tokyo
* These authors contributed equally

Capítulos

Resumo
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

在这里，我们提出了一种用于冷冻保存单细胞胚胎的改良方法，以及一种用于使用冷冻解冻胚胎和电穿孔以有效生成转基因小鼠的协议。

Tags

遗传学， 第158期， CRISPR/Cas9， 单细胞胚胎冷冻保存， 电穿孔， 转基因小鼠， 转基因， 基因组编辑， FBS
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter