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Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes
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Capítulos
- 00:00Introduction
- 00:54Synthesis of the UAA: Propargyl-L-Lysine (Prk)
- 04:46Production of the Recombinant Protein modified by PrK
- 08:13Removal of the Histidine Tag by TEV Protease Digestion
- 09:48Assessment of PrK Accessibility and Functionality and Conjugation to the Hapten
- 11:26Results: Efficient Incorporation of the PrK and Click Conjugation
- 12:49Conclusion
Genetic code expansion is applied for the introduction of an unnatural amino acid bearing a biorthogonal functional group on a carrier protein at a defined site. The biorthogonal function is further used for the site-selective coupling of a carbohydrate antigen to provide a homogeneous glycoconjugate vaccine.
