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Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ
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Biologia
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JoVE Journal Biologia
Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

DOI:
10.3791/60859-v

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10:05 min

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May 08, 2020

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, , , ,
1Ramón y Cajal Institute for Health Research (IRYCIS),Ramón y Cajal University Hospital, 2Centro Integrativo de Biología y Química Aplicada (CIBQA),Universidad Bernardo O’Higgins, 3Department of Molecular Biology, Faculty of Sciences,Universidad Autónoma de Madrid (UAM), 4Instituto de Investigaciones Biosanitarias, Facultad de Ciencias Experimentales,Universidad Francisco de Vitoria, 5Department of Radiology, Rehabilitation & Physiotherapy, Faculty of Medicine,Complutense University

Capítulos

  • 00:04Introduction
  • 01:26Hair Growth and Burn Induction and Long‐Term 5‐bromo‐2 -deoxyuridine (BrdU) Label Retaining Cell (LRC) Generation
  • 02:58Transient Nonlethal Reactive Oxygen Species (ROS) Production
  • 04:37BrdU Detection in Mouse Tail Skin
  • 05:38ROS Detection in Mouse Tail Skin
  • 07:16Results: Effects of Photodynamic mALA Treatment and Transient ROS Production
  • 09:00Conclusion

Summary

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Tadução automática

The aim of this protocol is to induce transient in vivo production of nonlethal levels of reactive oxygen species (ROS) in mouse skin, further promoting physiological responses in the tissue.

Tags

Keywords: Stem Cell NicheReactive Oxygen SpeciesROSProtoporphyrin IXPhotodynamic TherapySkinHair GrowthWound HealingBrdULabelingIn Vivo

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