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Immunology and Infection

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JoVE Journal Immunology and Infection
Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against Bacillus anthracis and Other Encapsulated Pathogens
 

Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against Bacillus anthracis and Other Encapsulated Pathogens

Article DOI: 10.3791/60873-v • 13:47 min • May 19th, 2020
May 19th, 2020 •
*1, *1, 1,3, 2
1Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, 2Headquarters Division, United States Army Medical Research Institute of Infectious Diseases, 3Bioqual
* These authors contributed equally

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Resumo
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The opsono-adherence assay is an alternative method to the opsono-phagocytic killing assay to evaluate the opsonic functions of antibodies in vaccine development.

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Keywords: Opsono-adherence Assay Functional Antibodies Vaccine Development Bacillus Anthracis Encapsulated Pathogens RAW 264.7 Cells Bacillus Anthracis Capsule Biosafety Level 2 India Ink Staining Bacterial Suspension
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