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Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine
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JoVE Journal Neurociência
Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine

Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine

DOI:
10.3791/60886-v

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06:40 min

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April 23, 2020

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, , ,
1Department of Psychology,Elizabethtown College, 2Department of Psychiatry,Yale University, 3Department of Cellular and Molecular Physiology,Yale University

Capítulos

  • 00:04Introduction
  • 00:56Electrode Implantation
  • 03:12Carbon Fiber and Stimulating Electrode/Guide Cannula Location Optimization
  • 04:26Combination Infusion and Stimulation Fast-Scan Cyclic Voltammetry (FSCV) Recording
  • 05:18Results: Representative Analyses of Stimulation-Evoked Phasic Dopamine Release
  • 06:03Conclusion

Summary

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The goal of this protocol is to directly manipulate ventral tegmental area receptors to study their contribution to subsecond dopamine release.

Tags

Keywords: Ventral Tegmental AreaPhasic Dopamine ReleaseNucleus AccumbensReceptor FunctionCarbon Fiber MicroelectrodeBipolar StimulatorReference ElectrodeSterilizedLambdaBregmaScrewDuraBipolar Stimulating Electrode

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