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内塞里亚淋病的金属有限生长，用于金属反应基因特征和从宿主配体中获取金属

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands

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内塞里亚淋病的金属有限生长，用于金属反应基因特征和从宿主配体中获取金属

DOI:

10.3791/60903-v

•

07:26 min

•

March 04, 2020

•

Stavros Maurakis, Cynthia Nau Cornelissen

¹Institute for Biomedical Sciences,Georgia State University

Capítulos

00:05Introduction
00:31Metal Limited Neisseria gonorrhoeae Growth
01:50Metal Loading
02:36Metal-Limited Growth Assay
03:50Outer Membrane Metal Transporter Ligand Binding Detection
05:38Results: Representative Differential Metal Responsive Protein Production, Growth-Restriction, and Binding
06:50Conclusion

Summary

Tadução automática

我们在这里描述了一种在金属受限液体介质中生长淋病的方法，以促进金属吸走基因的表达。我们还概述了下游实验，以表征在这些条件下生长的淋球菌的表型。这些方法可以适应其他细菌中金属反应基因的表征。

Tags

Metal-limited Growth Neisseria Gonorrhoeae Metal-responsive Genes Metal Acquisition Host Ligands Nutritional Immunity Metal Restricted Conditions Bacterial Growth CDM Klett Colorimeter Metal Loaded Proteins Transferrin Dialysis 96 Well Microplate Optical Density

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