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Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
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JoVE Journal Genética
Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

DOI:
10.3791/60905-v

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07:29 min

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May 16, 2020

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, , ,
1Department of Molecular Developmental Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences,Radboud University, Nijmegen, The Netherlands, 2Department of Dermatology, Radboud University Medical Center,Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands, 3Department of Human Genetics,Radboud University Medical Center, Nijmegen, The Netherlands

Capítulos

  • 00:04Introduction
  • 01:10Human Primary Keratinocyte Differentiation by Contact Inhibition
  • 02:20RNA-seq Data Analysis
  • 04:53Results: Keratinocyte Differentiation and Analysis
  • 06:47Conclusion

Summary

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Presented here is a stepwise procedure for in vitro differentiation of human primary keratinocytes by contact inhibition followed by characterization at the molecular level by RNA-seq analysis.

Tags

In VitroKeratinocyte DifferentiationRNA-seq2D MonolayerProliferation MediumDifferentiation MediumCell Seeding DensityRNA IsolationCDNA ConversionLibrary PreparationNext-generation SequencingRead MappingDESeq2RLD NormalizationVST Normalization

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