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1Center for Biological Systems Analysis,University of Freiburg, 2Faculty of Biology,University of Freiburg, 3Freiburg Institute for Advanced Studies (FRIAS),University of Freiburg, 4BIOSS Centre for Biological Signalling Studies,University of Freiburg, 5IMTEK Department of Microsystems Engineering,University of Freiburg, 6Cluster of Excellence livMatS at FIT – Freiburg Center for Interactive Materials and Bioinspired Technologies,University of Freiburg
Capítulos
- 00:05Introduction
- 00:45Protein Expression and Preparation
- 03:38Dye-modification of Elastin-like Proteins via SPAAC
- 04:44THF Swelling Protocol
- 06:29BuOH Extrusion Protocol
- 07:19Results: THF Swelling Method and the BuOH Extrusion Method for the Guided Self-assembly of Amphiphilic ELPs into Supramolecular Structures
- 09:22Conclusion
À l’interface des solvants organiques et aqueux, les protéines amphiphiliques sur mesure de type élastine s’assemblent en structures supramoléculaires complexes telles que les vésicules, les fibres et les coacervates déclenchés par des paramètres environnementaux. Les protocoles d’assemblage décrits donnent des compartiments à base de membrane protéique (PMBCs) avec des propriétés de thon, permettant l’encapsulation de diverses cargaisons.
