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Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis

Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis

DOI:

10.3791/60938-v

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08:41 min

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October 07, 2020

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Doğacan Yücel*^1,2, Jacob Solinsky*², Jop H. van Berlo^2,3

¹Department of Integrative Biology and Physiology,University of Minnesota, ²Lillehei Heart Institute, Department of Medicine,University of Minnesota, ³Stem Cell Institute,University of Minnesota

Capítulos

00:04Introduction
00:50Perfusion and Fixation of the Heart
01:52Isolation of Fixed Cardiomyocytes
03:17Image Quantification and Data Analysis
04:53Data Analysis
06:25Results: Segmentation and Classification of Isolated Cardiomyocytes
08:19Conclusion

Summary

Tadução automática

The goal of this work is to develop a method to reproducibly isolate cardiomyocytes from the adult heart and measure DNA content and nucleation.

Tags

Keywords: Cardiomyocytes Isolation Immunocytochemistry Ploidy Analysis Fixed Hearts Perfusion Collagenase Dissociation Filtration Centrifugation

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