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Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

DOI:
10.3791/60939-v

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09:09 min

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April 12, 2020

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, , , , , ,
1Dept. of Neurosurgery,University of Michigan Medical School, 2Dept. of Cell and Developmental Biology,University of Michigan Medical School, 3Dept. of Ophthalmology & Visual Science,University of Michigan Medical School, 4Rogel Cancer Center,University of Michigan Medical School

Capítulos

  • 00:05Introduction
  • 01:17Animal Perfusion and Brain Preservation
  • 02:01Glioma Brain Tumors Tissue Cryopreservation
  • 02:52Frozen Brain Tumor Tissue Sectioning
  • 03:58Cryopreserved Brain Tissue Section Fixation and Staining
  • 04:48Laser Capture Microdissection
  • 06:30Results: Representative RNA Quality Control and Library Preparation
  • 07:55Conclusion

Summary

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Laser microdissection (LMD) is a sensitive and highly reproducible technique that can be used to uncover pathways that mediate glioma heterogeneity and invasion. Here, we describe an optimized protocol to isolate discrete areas from glioma tissue using laser LMD followed by transcriptomic analysis.

Tags

Laser MicrodissectionRNA SequencingIntratumor HeterogeneityMesenchymal CellsTumor InvasionTumor MorphologyMolecular ProfilesTumor ProgressionTherapeutic TargetsBioluminescencePerfusionCryopreservationSpatial CharacterizationMolecular Characterization

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