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Immunology and Infection

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JoVE Journal Immunology and Infection
Determination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay
 

Determination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay

Article DOI: 10.3791/60985-v • 09:49 min • April 29th, 2020
April 29th, 2020 •
*1,2, *1,2, 1,2, 1,2, 1,2, 1,2
1Department of Biological Sciences, Clemson University, 2Eukaryotic Pathogens Innovation Center, Clemson University
* These authors contributed equally

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Resumo
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Presented here is a protocol to evaluate the inhibition efficacy of chemical compounds against in vitro intracellular growth of Toxoplasma gondii using a luciferase-based growth assay. The technique is used to confirm inhibition specificity by genetic deletion of the corresponding target gene. The inhibition of LHVS against TgCPL protease is evaluated as an example.

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Keywords: Toxoplasma Gondii Luciferase-based Growth Assay Chemical Inhibitor Drug Target CRISPR-Cas9 Intracellular Pathogen High-throughput Screening Parasite Doubling Time Human Foreskin Fibroblasts Luciferase Substrate Microplate Reader Normalized Fold-change Linear Regression
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