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1Hepatic Fibrosis Group,QIMR Berghofer Medical Research Institute, 2School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute,Curtin University, 3School of Veterinary and Life Sciences,Murdoch University, 4School of Medicine,The University of Queensland, 5Madrid Institute for Advanced Studies (IMDEA) in Food, CEI UAM+CSIC, 6School of Medicine,Griffith University, 7School of Biological Sciences,Queen’s University Belfast, 8Department of Gastroenterology & Hepatology,Fiona Stanley and Fremantle Hospital Group, 9School of Medical and Health Sciences,Edith Cowan University, 10Centre for Cell Therapy and Regenerative Medicine, School of Biomedical Sciences,University of Western Australia
Capítulos
- 00:00Introduction
- 01:23Setup of the Vibratome
- 03:15Liver Removal and Preparation
- 06:07Production of Liver Slices
- 10:14Results: Viability, Collagen, and Effects of Selected Bile Acids
- 11:45Conclusion
Bu protokol farelerden canlı hassas kesilmiş karaciğer dilimleri üretimi için basit ve güvenilir bir yöntem sağlar. Ex vivo doku örnekleri laboratuvar doku kültürü koşullarında birden fazla gün boyunca muhafaza edilebilir, karaciğer patobiyolojisi incelemek için esnek bir model sağlayan.
