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Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish
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Capítulos
- 00:04Introduction
- 00:54Fluorescently Labeling Cells with DiI(1,1 -Dioctadecyl-3,3,3 ,3 -Tetramethylindocarbocyanine Perchlorate)
- 02:10Microinjecting Zebrafish Larvae
- 04:37Setting up Drug Screen with Xenografted Zebrafish
- 06:22Imaging Xenografted Zebrafish and Quantifying Fluorescence
- 08:36Results: Xenografted Tumor Area
- 09:22Conclusion
Zebrafish xenograft models allow for high-throughput drug screening and fluorescent imaging of human cancer cells in an in vivo microenvironment. We developed a workflow for large scale, automated drug screening on patient-derived leukemia samples in zebrafish using an automated fluorescence microscope equipped imaging unit.
