/
/
Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Introduction
- 00:36U-118MG Glioblastoma Cell Line Culture and Electroporation
- 02:13Real-Time Cell Analysis System, Cell Invasion and Migration (CIM) Plate, and Electroporated U-118MG Cells Preparation
- 04:59Baseline Reading, Cell Seeding, and Cell Impedance Measurement and Visualization
- 06:43Results: Representative Effects of Crk/CrkL Knockdown on U-118MG Cell Migration
- 08:48Conclusion
Cancer is a lethal disease due to its ability to metastasize to different organs. Determining the ability of cancer cells to migrate and invade under various treatment conditions is crucial to assessing therapeutic strategies. This protocol presents a method to assess the real-time metastatic abilities of a glioblastoma cancer cell line.
