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Measurements of Physiological Stress Responses in C. Elegans
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Biologia
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JoVE Journal Biologia
Measurements of Physiological Stress Responses in C. Elegans

Measurements of Physiological Stress Responses in C. Elegans

DOI:
10.3791/61001-v

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10:36 min

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May 21, 2020

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, , , , , , ,
1Department of Molecular and Cell Biology,University of California, Berkeley

Capítulos

  • 00:04Introduction
  • 01:00Stress Response Induction: hsp-4p::GFP Readout
  • 02:11Stereo Microscope or Low-Magnification Wide-Field/Compound Microscope Imaging
  • 04:33Quantitative Reporter Measurement
  • 06:54Stress and Heat Sensitivity Measurement
  • 07:54Results: Representative Cellular Stress Response Evaluation
  • 09:56Conclusion

Summary

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Here, we characterize cellular proteotoxic stress responses in the nematode C. elegans by measuring the activation of fluorescent transcriptional reporters and assaying sensitivity to physiological stress.

Tags

C. ElegansPhysiological Stress ResponsesStress Response AssayHeat Shock ProteinEndoplasmic Reticulum Unfolded Protein ResponseGFP ReporterSodium AzideMicromanipulationHigh-throughputGenetic PerturbationsPharmacological PerturbationsMolecular And Physiological AnalysisSynchronizationImagingMicroscopy

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