/
/
Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:04Introduction
- 00:40Preparation of Mouse Brain Membranes
- 02:29Acyl-PEGyl Exchange Gel-Shift (APEGS) Assay
- 06:54Western Blot Analysis of PEGylated Proteins
- 07:11Results: Palmitoylation of SynDIG Proteins
- 07:41Conclusion
Palmitoylation entails the incorporation of a 16-carbon palmitate moiety to cysteine residues of target proteins in a reversible manner. Here, we describe a biochemical approach, the acyl-PEGyl exchange gel shift (APEGS) assay, to investigate the palmitoylation state of any protein of interest in mouse brain lysates.
