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Omvendt transskription Loop-medieret Isothermal Amplification (RT-LAMP) Assay for den specifikke og hurtige påvisning af Tilapia Lake Virus
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JoVE Journal Imunologia e Infecção
Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus

Omvendt transskription Loop-medieret Isothermal Amplification (RT-LAMP) Assay for den specifikke og hurtige påvisning af Tilapia Lake Virus

DOI:
10.3791/61025-v

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07:47 min

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May 18, 2020

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, , , ,
1Department of Biotechnology, Faculty of Applied Science,King Mongkut’s University of Technology North Bangkok (KMUTNB), 2Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine,Kasetsart University, 3Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies,Kasetsart University, 4Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS),King Mongkut’s University of Technology North Bangkok (KMUTNB)

Capítulos

  • 00:03Introduction
  • 00:59RNA Extraction
  • 02:50Primer Design
  • 05:20Demonstration of RT-Lamp Assay
  • 07:10Resultados
  • 08:19Conclusion

Summary

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Vi præsenterer en RT-LAMP analyse til påvisning af TiLV i tilapia fisk ved hjælp af enkle instrumenter over en relativt kort periode i forhold til konventionelle RT-PCR teknikker. Denne protokol kan bidrage til at kontrollere den epidemiske spredning af TiLVD, især i udviklingslandene.

Tags

RT-LAMPReverse Transcription Loop-mediated Isothermal AmplificationTilapia Lake VirusTiLVRapid DetectionRNA ExtractionPrimer DesignPrimerExplorerLAMP PrimersSegment 3AquacultureTilapiaVirus Detection

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