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Measuring Real-time Drug Response in Organotypic Tumor Tissue Slices
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JoVE Journal Pesquisa do Câncer
Measuring Real-time Drug Response in Organotypic Tumor Tissue Slices

Measuring Real-time Drug Response in Organotypic Tumor Tissue Slices

DOI:
10.3791/61036-v

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08:27 min

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May 02, 2020

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, ,
1Division of Human Biology,Fred Hutchinson Cancer Research Center, 2Department of Molecular and Cellular Biology,University of Washington, 3Department of Pharmacology,University of Washington

Capítulos

  • 00:04Introduction
  • 01:12Tumor Tissue Slice Preparation
  • 04:13Viability Measurement
  • 05:07Drug Effect Evaluation
  • 05:58Results: Representative Tissue Slice Viability Measurement and Drug Response
  • 07:33Conclusion

Summary

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We introduce a protocol for measuring real-time drug response in organotypic tumor tissue slices. The experimental strategy outlined here provides a platform to carry out medium-high throughput drug screens on tissue slices derived from clinical or mouse tumors in ex vivo conditions.

Tags

Real-time Drug ResponseViability AssessmentMedium Throughput ScreeningPre-clinical Drug DevelopmentOncologyCancer BiologyNeurociênciaBiologia do DesenvolvimentoVibratomeBiopsy PunchesCyanoacrylate Glue

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