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Imunologia e Infecção
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Production and Characterization of Human Macrophages from Pluripotent Stem Cells
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Production and Characterization of Human Macrophages from Pluripotent Stem Cells

Production and Characterization of Human Macrophages from Pluripotent Stem Cells

DOI:
10.3791/61038-v

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08:05 min

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April 16, 2020

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, , , , , , ,
1Centre for Regenerative Medicine, Scottish Centre for Regenerative Medicine,University of Edinburgh, 2Centre for Reproductive Health, The Queen’s Medical Research Institute,University of Edinburgh

Capítulos

  • 00:04Introduction
  • 00:51Passaging Human iPSCs
  • 01:46Human iPSC Differentiation to Macrophages
  • 04:33High-Throughput Phagocytosis Assay
  • 05:07Results: Phenotype and Function of iPSC-Derived Macrophages
  • 07:08Conclusion

Summary

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This protocol describes the robust generation of macrophages from human induced pluripotent stem cells, and methods for their subsequent characterization. Cell surface marker expression, gene expression, and functional assays are used to assess the phenotype and function of these iPSC-derived macrophages.

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Keywords: MacrophagePluripotent Stem CellsCell TherapyGenetic ManipulationCell CultureCell DifferentiationEmbryoid BodiesCell PassagingCell HarvestingMedia Replenishment

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