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简化反向遗传学方法，恢复重组性轮状病毒表达报告蛋白

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

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简化反向遗传学方法，恢复重组性轮状病毒表达报告蛋白

DOI:

10.3791/61039-v

•

11:40 min

•

April 17, 2020

•

Asha A. Philip, Jin Dai, Sarah P. Katen, John T. Patton

¹Department of Biology,Indiana University

Capítulos

00:04Introduction
00:32Generation of Recombinant Virus
04:00Plaque Isolation of Recombinant Viruses
07:02Gel Electrophoresis of Viral dsRNA
09:11Results: Characteristics of the Recombinant Strains rSA11/wt and rSA11/NSP3-2A-3xFL-UnaG
10:58Conclusion

Summary

Tadução automática

从质粒DNA生成重组性轮状病毒为研究轮状病毒复制和发病机制以及发展轮状病毒表达载体和疫苗提供了重要工具。在这里，我们描述了一种简化的反向遗传学方法来产生重组性轮状病毒，包括表达荧光报告蛋白的菌株。

Tags

Reverse Genetics Recombinant Rotavirus Reporter Proteins BHKT7 Cells MA104 Cells Transfection Trypsin Plasmid Mixture Fluorescent Marker Proteins

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