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双电穿孔，以定时和空间分离的细胞群
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Neurociência
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JoVE Journal Neurociência
Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations
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双电穿孔，以定时和空间分离的细胞群

DOI:
10.3791/61046-v

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10:45 min

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June 14, 2020

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, , ,
1Estructura de Recerca Interdisciplinar en Biotecnología y Biomedicina (ERI BIOTECMED), Departamento de Biología Celular, Biología Funcional y Antropología Física,Universidad de Valencia

Capítulos

  • 00:04Introduction
  • 01:09First In Utero Electroporation Surgery
  • 03:20Second In Utero Electroporation
  • 04:18Tissue Harvesting and Sectioning: Strategy A
  • 06:09Tissue Harvesting and Sectioning: Strategy B
  • 07:24Confocal Fluorescence Imaging and Analysis
  • 08:34Results: Representative Double Electroporation-Facilitated Cell Interaction Visualization Strategies
  • 09:55Conclusion

Summary

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Tadução automática

子宫电穿孔的双倍允许瞄准空间和时间分离的细胞群。该技术可用于在正常条件下使用荧光蛋白可视化这些细胞群之间的相互作用，而且在功能实验后也可用于干扰感兴趣的基因。

Tags

Double In Utero ElectroporationCorticogenesisNeuron CellsNeuronal DisordersAutismSchizophreniaLateral VentriclesFast Green DyePlasmid DNAAnesthetized Pregnant MouseRazor70% EthanolIodine WipesRing ForcepsMouth Controlled Aspirator TubeLateral VentricleSquare-wave ElectroporaterCortical HemLateral Cortex

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