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Imunologia e Infecção
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Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

DOI:
10.3791/61049-v

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09:25 min

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May 24, 2020

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, , , ,
1Department of Medial Microbiology and Immunology,School of Medicine, University of California Davis, 2Department of Microbiology and Immunology,University of Texas Medical Branch at Galveston

Capítulos

  • 00:04Introduction
  • 01:03Recombination Vector Generation
  • 03:47Recombinant Virus Generation
  • 06:31Results: Representative Recombinant Poxvirus Generation
  • 08:36Conclusion

Summary

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This is a method to generate “scarless” recombinant vaccinia viruses using host-range selection and visual identification of recombinant viruses.

Tags

Recombinant PoxvirusesHost Range SelectionFluorescent MarkersHomologous RecombinationTransient Dominant SelectionVaccinia VirusForeign Gene ExpressionVaccinesPCRDNA Gel ExtractionDNA AssemblyE. Coli Transformation

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