Journal
/
Imunologia e Infecção
/
Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis

Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis

DOI:
10.3791/61050-v

•

09:01 min

•

November 17, 2020

•

, , , ,
1Translational Medicine Research Center, Ruijin Hospital North,Shanghai Jiao Tong University School of Medicine, 2Department of Microbiology and Immunology,Tongji University School of Medicine, 3Shanghai Institute of Immunology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation,Shanghai Jiao Tong University School of Medicine

Capítulos

  • 00:04Introduction
  • 00:53C57BL/6 Mouse Immunization
  • 01:58Single Brain Cell Suspension Preparation
  • 04:19Flow Cytometric Analysis
  • 07:00Results: Representative Experimental Autoimmune Encephalomyelitis (EAE) Course and Brain Lymphocyte Analyses
  • 08:22Conclusion

Summary

Tadução automática

Tadução automática

This manuscript presents a protocol to induce active experimental autoimmune encephalomyelitis (EAE) in mice. A method for the isolation and characterization of the infiltrated lymphocytes in the central nervous system (CNS) is also presented to show how lymphocytes are involved in the development of CNS autoimmune disease.

Tags

Keywords: Flow CytometryLymphocyte InfiltrationCentral Nervous SystemExperimental Autoimmune EncephalomyelitisImmune CellsMOG 35-55Pertussis ToxinNeurological Signs

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/61050?article_type=v

Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge
check_url/pt/61050?article_type=v

Culturing Microglia from the Neonatal and Adult Central Nervous System
check_url/pt/61050?article_type=v

Myelin Oligodendrocyte Glycoprotein (MOG35-55) Induced Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6 Mice
check_url/pt/61050?article_type=v

Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood
check_url/pt/61050?article_type=v

Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry
check_url/pt/61050?article_type=v

Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking
check_url/pt/61050?article_type=v

A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System
check_url/pt/61050?article_type=v

Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo
check_url/pt/61050?article_type=v

Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells
check_url/pt/61050?article_type=v

Adjuvant Activity of Mycobacterium paratuberculosis in Enhancing the Immunogenicity of Autoantigens During Experimental Autoimmune Encephalomyelitis (Video) | JoVE

Read Article