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实验自身免疫性脑脊髓炎中枢神经系统淋巴细胞渗透的流量细胞计量分析

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis

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实验自身免疫性脑脊髓炎中枢神经系统淋巴细胞渗透的流量细胞计量分析

DOI:

10.3791/61050-v

•

09:01 min

•

November 17, 2020

•

Zhe Ji², Chenghua Zhou, Hongshen Niu, Jinshen Wang, Lei Shen

¹Translational Medicine Research Center, Ruijin Hospital North,Shanghai Jiao Tong University School of Medicine, ²Department of Microbiology and Immunology,Tongji University School of Medicine, ³Shanghai Institute of Immunology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation,Shanghai Jiao Tong University School of Medicine

Capítulos

00:04Introduction
00:53C57BL/6 Mouse Immunization
01:58Single Brain Cell Suspension Preparation
04:19Flow Cytometric Analysis
07:00Results: Representative Experimental Autoimmune Encephalomyelitis (EAE) Course and Brain Lymphocyte Analyses
08:22Conclusion

Summary

Tadução automática

本手稿提供了在小鼠中诱导活性实验性自身免疫性脑脊髓炎（EAE）的协议。还提出了一种分离和表征中枢神经系统（CNS）中渗透淋巴细胞的方法，以表明淋巴细胞如何参与中枢自身免疫性疾病的发展。

Tags

Keywords: Flow Cytometry Lymphocyte Infiltration Central Nervous System Experimental Autoimmune Encephalomyelitis Immune Cells MOG 35-55 Pertussis Toxin Neurological Signs

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