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JoVE Journal Biochemistry
A Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Platform for Investigating Peptide Biosynthetic Enzymes
 

A Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Platform for Investigating Peptide Biosynthetic Enzymes

Article DOI: 10.3791/61053-v • 11:32 min • May 4th, 2020
May 4th, 2020 •
1, 1
1Department of Chemistry, McGill University

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Lanthipeptide synthetases catalyze multistep reactions during the biosynthesis of peptide natural products. Here, we describe a continuous, bottom-up, hydrogen-deuterium exchange mass spectrometry (HDX-MS) workflow that can be employed to study the conformational dynamics of lanthipeptide synthetases, as well as other similar enzymes involved in peptide natural product biosynthesis.

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Keywords: Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Protein Conformational Dynamics Protein Of Interest Quench Buffer Bottom-up Liquid Chromatography Mass Spectrometry Proteomic Software Program FASTA Format Electrospray MSE Glucan One Fiber No Peptide B Nonspecific Digest Carbamidomethyl C Apex 3D Peptide 3D Ion Accounting
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