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通过预置等电聚焦（IEF）方法，将生物活性小分子、肽与天然来源和蛋白质从微生物中分离出来

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method

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通过预置等电聚焦（IEF）方法，将生物活性小分子、肽与天然来源和蛋白质从微生物中分离出来

DOI:

10.3791/61101-v

•

09:57 min

•

June 14, 2020

•

Raja Veerapandian, Anuja Paudyal, Adeline Chang, Govindsamy Vediyappan

¹Division of Biology,Kansas State University

Capítulos

00:04Introduction
00:45Setting Up IEF Unit
02:55IEF and Gymnema sylvestre
04:17IEF and C. albicans
06:45Gymnema sylvestre Bioactivity
07:36Results: Isolating Proteins, Peptides, and Small Molecules
09:21Conclusion

Summary

Tadução automática

目标是通过使用液相同电聚焦（IEF）方法，分馏和分离生物活性小分子、复杂植物提取物中的肽和致病微生物中的蛋白质。此外，分离的分子被识别，其生物活性得到确认。

Tags

Keywords: Isoelectric Focusing Separation Purification Small Molecules Peptides Proteins Microbes Natural Sources Therapeutic Plant Extract Gymnema Sylvestre Ion Exchange Membranes Electrophoresis Cooling Fraction Collector Centrifugation

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