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使用hiPSC衍生心肌细胞的缺血性心脏病模型和基于视频的心肌细胞收缩比较

JoVE Journal
Medicina

This content is Free Access.

JoVE Journal Medicina

Model of Ischemic Heart Disease and Video-Based Comparison of Cardiomyocyte Contraction Using hiPSC-Derived Cardiomyocytes

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使用hiPSC衍生心肌细胞的缺血性心脏病模型和基于视频的心肌细胞收缩比较

DOI:

10.3791/61104-v

•

05:06 min

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May 05, 2020

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Yun Liu*¹, Yin Liang*¹, Mengxue Wang*¹, Chen Wang, Heng Wei, Keiji Naruse, Ken Takahashi

¹Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,Okayama University, ²Institute of Laboratory Animals, Graduate School of Medicine,Kyoto University

Capítulos

00:04Introduction
01:05Human Induced Pluripotent Stem Cell (hiPSC) Cardiac Differentiation Induction
02:30Induced Pluripotent Stem Cell-Cardiomyocyte (iPS-CM) Contractility Assessment
03:15Ischemia Exposure
03:42Results: Representative Cardiac Cell Differentiation
04:27Conclusion

Summary

Tadução automática

我们提出了一个缺血性心脏病的模型，使用从人类诱导多能干细胞中提取的心肌细胞，以及一种定量评估缺血引起的组织损伤的方法。该模型可为药物筛查和进一步研究缺血性心脏病提供一个有用的平台。

Tags

IPS Cells Cardiomyocytes Ischemic Heart Disease Video Analysis Cardiomyocyte Contraction Human Heart Physiology Personalized Drug Screening HiPSC-derived Cardiomyocytes Particle Image Velocimetry Phase Contrast Microscopy

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