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Live-Cell Imaging af livscyklus bakteriel Predator Bdellovibrio bacteriovorus ved hjælp af Time-Lapse Fluorescens mikroskopi
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Biologia
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JoVE Journal Biologia
Live-Cell Imaging of the Life Cycle of Bacterial Predator Bdellovibrio bacteriovorus using Time-Lapse Fluorescence Microscopy

Live-Cell Imaging af livscyklus bakteriel Predator Bdellovibrio bacteriovorus ved hjælp af Time-Lapse Fluorescens mikroskopi

DOI:
10.3791/61105-v

•

08:56 min

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May 08, 2020

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, ,
1Department of Molecular Microbiology, Faculty of Biotechnology,University of Wroclaw

Capítulos

  • 00:04Introduction
  • 00:34B. bacteriovorus Lysate Preparation
  • 01:47Host Cell Preparation
  • 02:32Time-Lapse Fluorescence Microscopy Layout Preparation
  • 03:36Time-Lapse Fluorescence Microscopy
  • 05:30Time-Lapse Image Processing and Movie Generation
  • 07:11Results: Representative B. bacteriovorus Life Cycle Captured by Time-Lapse Fluorescence
  • 08:16Conclusion

Summary

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Præsenteret her er en protokol, der beskriver overvågning af hele livscyklus aggressiv bakterie Bdellovibrio bakterien ved hjælp af time-lapse fluorescens mikroskopi i kombination med en agarose pad og celle-imaging retter.

Tags

Keywords: Live-cell ImagingBdellovibrio BacteriovorusTime-lapse Fluorescence MicroscopyBacterial PredatorLife CyclePrey CellsAgarose PadCalcium HEPES BufferOptical DensityCentrifugationFluorescence MicroscopyPrey LysisMulti-attack Phase

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