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Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
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JoVE Journal Química
Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay

Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay

DOI:
10.3791/61122-v

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06:19 min

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May 12, 2020

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,
1School of Molecular Sciences, The Biodesign Institute – Center for Personalized Diagnostics,Arizona State University

Capítulos

  • 00:04Introduction
  • 01:08Reagent Preparation
  • 02:26TBARS Assay
  • 03:56Results: Thiobarbituric Acid Reactive Substances Assay
  • 05:30Conclusion

Summary

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The goal of the thiobarbituric acid reactive substances assay is to assess oxidative stress in biological samples by measuring the production of lipid peroxidation products, primarily malondialdehyde, using visible wavelength spectrophotometry at 532 nm. The method described here can be applied to human serum, cell lysates, and low density lipoproteins.

Tags

Keywords: TBARS AssayOxidative StressBiological SamplesMDAThiobarbituric AcidSodium HydroxideSodium AcetateSpectrophotometric AnalysisSample PreparationStandard Curve

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