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骨骼肌成纤维细胞外泌体的分离和表征

JoVE Journal
Biologia

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JoVE Journal Biologia

Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts

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骨骼肌成纤维细胞外泌体的分离和表征

DOI:

10.3791/61127-v

•

06:27 min

•

May 16, 2020

•

Diantha van de Vlekkert, Xiaohui Qiu, Ida Annunziata, Alessandra d’Azzo

¹Department of Genetics,St. Jude Children’s Research Hospital

Capítulos

00:05Introduction
00:45Primary Fibroblast Isolation and Culture
03:04Purification of Exosomes Using Differential and Ultra-Centrifugation
04:58Results: Characterization of Purified Exosomes
05:47Conclusion

Summary

Tadução automática

该方案说明了1）从成年小鼠腓肠肌中分离和培养原代成纤维细胞，以及2）使用差异超速离心法结合蔗糖密度梯度，然后进行蛋白质印迹分析的外泌体纯化和表征。

Tags

Keywords: Exosomes Skeletal Muscle Fibroblasts Differential Centrifugation Isolation Characterization Biomarkers Cell Culture Low Oxygen Conditions

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