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Imunologia e Infecção
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Production of Germ-Free Fast-Growing Broilers from a Commercial Line for Microbiota Studies
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Production of Germ-Free Fast-Growing Broilers from a Commercial Line for Microbiota Studies

Production of Germ-Free Fast-Growing Broilers from a Commercial Line for Microbiota Studies

DOI:
10.3791/61148-v

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05:51 min

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June 18, 2020

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, , , , , , , , ,
1INRAE, PFIE, UE-1277, 2INRAE, ISP, Université de Tours

Capítulos

  • 00:05Introduction
  • 00:50Isolator Preparation and Egg Collection
  • 02:06Hatching
  • 02:52Bacteriologic Status Analysis
  • 03:54Results: Viable Eggs from the Hatching Experiments
  • 05:08Conclusion

Summary

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Here we describe a method for the generation of germ-free chicks from eggs of a commercial broiler line, the Ross PM3. This method can be adapted for the generation of germ-free animals from other poultry species.

Tags

Keywords: Germ-freeFast-growing BroilersCommercial LineMicrobiota StudiesIsolatorSterile ConditionsPeracetic AcidEmbryonated EggsHatchingFecal SampleThioglycolate BrothBrain Heart Infusion

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