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卡诺哈布迪蒂斯 伊利根斯新蛋白合成率评估
JoVE Journal
Biologia
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JoVE Journal Biologia
Assessment of de novo Protein Synthesis Rates in Caenorhabditis elegans
DOI:

06:27 min

September 12, 2020

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Capítulos

  • 00:00Introduction
  • 00:42Maintenance, Synchronization and Preparation of Transgenic Nematodes for Monitoring de novo Protein Synthesis
  • 02:11FRAP Assay using Transgenic Animals Expressing Somatic Tissue Reporters plfe-2GFP and psod-3GFP
  • 03:01Mounting the Samples and Perform FRAP Assay using Transgenic Nematodes Expressing Pan-Neuronally Cytoplasmic GFP, punc-119GFP
  • 04:19Representative Results: In Vivo Assessment of de novo Protein Synthesis in C. elegans
  • 05:46Conclusion

Summary

Tadução automática

在这里,我们介绍和描述一种非放射性和非侵入性的方法,以评估体内的去新蛋白合成,利用线虫 卡诺 哈布迪炎和荧光漂白后恢复(FRAP)。该方法可与遗传和/或药理学屏幕相结合,识别蛋白质合成的新调制器。

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